Algorithmen der Bioinformatik


Real-time taxonomic and functional analysis of long reads on a laptop


Advances in mobile sequencing devices and laptop performancemake metagenomic sequencing and analysis in the field a technologically feasible prospect. However, metagenomic analysis pipelines are usually designed to run on servers and or in a compute cloud. 

MAIRA is a new standalone program for interactive taxonomic and functional analysis of long read metagenomic sequencing data on a laptop without internet access. The program performs fast, online, genus-level analysis, and on-demand, detailed taxonomic and functional analysis. It uses two levels of frame-shift-aware alignment of DNA reads against protein reference sequences, and then performs detailed analysis using a protein synteny graph.

We envision this software being used by researchers in the field, when access to servers or cloud facilities is difficult, or by individuals that do not routinely have access to such facilities, such as medical researchers, crop scientists, or teachers, say.
Download the software here.
Download the database files here.
Albrecht, B., Bagci, C and Huson, DH MAIRA- real-time taxonomic and functional analysis of long reads on a laptop. Submitted to APBC 2020.